NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster.
نویسندگان
چکیده
We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo-Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.
منابع مشابه
Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas. Corrigendum
The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal s...
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The molybdenum EXAFS of the Mo(2Fe-2S) protein from Desulfovibrio gigas has been examined using fluorescence detection and synchrotron radiation. In the oxidized form the molybdenum environment is found to contain two terminal oxo groups and two long (2.47 A) Mo-S bonds. Evidence was also found for an oxygen or nitrogen donor ligand at 1.90 A. Addition of dithionite to the oxidized enzyme resul...
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The characterization of a novel Mo-Fe protein (MorP) associated with a system that responds to Mo in DesulfoVibrio alaskensis is reported. Biochemical characterization shows that MorP is a periplasmic homomultimer of high molecular weight (260 ( 13 kDa) consisting of 16-18 monomers of 15321.1 ( 0.5 Da. The UV/visible absorption spectrum of the as-isolated protein shows absorption peaks around 2...
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A novel adenylate kinase (AK) has recently been purified from Desulfovibrio gigas and characterized as a Co(2+)/Zn(2+)-containing enzyme: this is an unusual characteristic for AKs from Gram-negative bacteria, in which these enzymes are normally devoid of metals. Here, we studied the conformational stability of holo- and apo-AK as a function of temperature by differential scanning calorimetry (D...
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ورودعنوان ژورنال:
- Biomolecular NMR assignments
دوره 1 1 شماره
صفحات -
تاریخ انتشار 2007